stempro-34 serum-free medium stempro-34 sfm (Thermo Fisher)
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stempro-34 serum-free medium stempro-34 sfm
Stempro 34 Serum Free Medium Stempro 34 Sfm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stempro-34+sfm+medium/serum+free+media+stempro+34+sfm/pmc12143994-63-16-21
Average 90 stars, based on 1 article reviews
Stempro 34 Serum Free Medium Stempro 34 Sfm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stempro-34+sfm+medium/serum+free+media+stempro+34+sfm/pmc12143994-63-16-21
Average 90 stars, based on 1 article reviews
stempro-34 serum-free medium stempro-34 sfm - by Bioz Stars,
2026-10
90/100 stars
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Isolation:Article Title: Characterization of an induced pluripotent stem cell line NCHi025-A from a 1-year-old female with pulmonary stenosis harboring a heterozygous HAND2 variant. Article Snippet: HAND2 is a transcription factor that plays a vital role in the development of the heart, limbs, and pharyngeal arch.. Functional defects in HAND2 have been shown to cause congenital malformations in the extremities of the body and the heart.. NCHi025-A iPSC line was generated from a 1-year-old female with pulmonary stenosis and harbors a novel de novo heterozygous variant of uncertain significance within HAND2 (NM_021973.3; c.247delG; p.Val83CysfsTer16). Article Title: No relationship between non-IgE-mediated mechanisms (complement activation or direct activation of mast cells and basophils) during diclofenac etalhyaluronate (SI-613/ONO-5704)-induced anaphylaxis Article Snippet: In brief, cells were cultured in StemPro-34 SFM medium supplemented with 2 mM Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation Article Snippet: The organoids were subsequently cultured in StemPro-34 SFM medium (10639011, Thermo Fisher Scientific) supplemented with 2% Article Title: Characterization of an induced pluripotent stem cell line (NCHi024-A) from a male infant with bicuspid aortic valve. Article Snippet: PBMCs were isolated and cultured for one week in StemPro-34 SFM medium (Thermo Fisher Scientific) supplemented with 100 ng/mL SCF (PeproTech), 20 ng/mL IL-3 (PeproTech), 100 ng/mL Article Title: Restoring mitochondrial function promotes hematopoietic reconstitution from cord blood following cryopreservation-related functional decline Article Snippet: After 24 h, the H5100 medium was replaced 630 with 200 μl erythro-myeloid differentiation medium containing StemPro-34 SFM 631 medium (Thermo Fisher) supplemented with the following cytokines (all from 632 Peprotech): SCF (100 ng/ml), Flt3-L (20 ng/ml), TPO (100 ng/ml), IL-6 (50 ng/ml), IL-633 3 (10 ng/ml), IL-11 (50 ng/ml), GM-CSF (20 ng/ml), IL-2 (10 ng/ml), IL-7 (20 ng/ml), 634 and EPO (3 units/ml), as well as 1% Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation. Article Snippet: The organoids were subsequently cultured in StemPro-34 SFM medium (10639011, Thermo Fisher Scientific) supplemented with 2% Article Title: Restoring mitochondrial function promotes hematopoietic reconstitution from cord blood following cryopreservation-related functional decline Article Snippet: After 24 hours, the H5100 medium was replaced with 200 μL erythro-myeloid differentiation medium containing StemPro-34 SFM medium (Thermo Fisher Scientific) supplemented with the following cytokines (all from Peprotech): SCF (100 ng/mL); FLT-3L (20 ng/mL); TPO (100 ng/mL); IL-6 (50 ng/mL); IL-3 (10 ng/mL); IL-11 (50 ng/mL); GM-CSF (20 ng/mL); IL-2 (10 ng/mL); IL-7 (20 ng/mL); and EPO (3 units/mL), as well as 1% Article Title: Adenine base editing rescues pathogenic phenotypes in tissue engineered vascular model of Hutchinson-Gilford progeria syndrome Article Snippet: To differentiate iPSCs to viECs, the cells were cultured in viEC induction media (Stempro-34 SFM medium (Thermo Fisher) supplemented with 200 ng/mL VEGF165 (Genscript) and 2 μ M forskolin (Sigma-Aldrich)) starting on day 4. Cell Culture:Article Title: Characterization of an induced pluripotent stem cell line NCHi025-A from a 1-year-old female with pulmonary stenosis harboring a heterozygous HAND2 variant. Article Snippet: HAND2 is a transcription factor that plays a vital role in the development of the heart, limbs, and pharyngeal arch.. Functional defects in HAND2 have been shown to cause congenital malformations in the extremities of the body and the heart.. NCHi025-A iPSC line was generated from a 1-year-old female with pulmonary stenosis and harbors a novel de novo heterozygous variant of uncertain significance within HAND2 (NM_021973.3; c.247delG; p.Val83CysfsTer16). Article Title: No relationship between non-IgE-mediated mechanisms (complement activation or direct activation of mast cells and basophils) during diclofenac etalhyaluronate (SI-613/ONO-5704)-induced anaphylaxis Article Snippet: In brief, cells were cultured in StemPro-34 SFM medium supplemented with 2 mM Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation Article Snippet: The organoids were subsequently cultured in StemPro-34 SFM medium (10639011, Thermo Fisher Scientific) supplemented with 2% Article Title: Characterization of an induced pluripotent stem cell line (NCHi024-A) from a male infant with bicuspid aortic valve. Article Snippet: PBMCs were isolated and cultured for one week in StemPro-34 SFM medium (Thermo Fisher Scientific) supplemented with 100 ng/mL SCF (PeproTech), 20 ng/mL IL-3 (PeproTech), 100 ng/mL Article Title: Restoring mitochondrial function promotes hematopoietic reconstitution from cord blood following cryopreservation-related functional decline Article Snippet: After 24 h, the H5100 medium was replaced 630 with 200 μl erythro-myeloid differentiation medium containing StemPro-34 SFM 631 medium (Thermo Fisher) supplemented with the following cytokines (all from 632 Peprotech): SCF (100 ng/ml), Flt3-L (20 ng/ml), TPO (100 ng/ml), IL-6 (50 ng/ml), IL-633 3 (10 ng/ml), IL-11 (50 ng/ml), GM-CSF (20 ng/ml), IL-2 (10 ng/ml), IL-7 (20 ng/ml), 634 and EPO (3 units/ml), as well as 1% Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation. Article Snippet: The organoids were subsequently cultured in StemPro-34 SFM medium (10639011, Thermo Fisher Scientific) supplemented with 2% Article Title: Restoring mitochondrial function promotes hematopoietic reconstitution from cord blood following cryopreservation-related functional decline Article Snippet: After 24 hours, the H5100 medium was replaced with 200 μL erythro-myeloid differentiation medium containing StemPro-34 SFM medium (Thermo Fisher Scientific) supplemented with the following cytokines (all from Peprotech): SCF (100 ng/mL); FLT-3L (20 ng/mL); TPO (100 ng/mL); IL-6 (50 ng/mL); IL-3 (10 ng/mL); IL-11 (50 ng/mL); GM-CSF (20 ng/mL); IL-2 (10 ng/mL); IL-7 (20 ng/mL); and EPO (3 units/mL), as well as 1% Article Title: Adenine base editing rescues pathogenic phenotypes in tissue engineered vascular model of Hutchinson-Gilford progeria syndrome Article Snippet: To differentiate iPSCs to viECs, the cells were cultured in viEC induction media (Stempro-34 SFM medium (Thermo Fisher) supplemented with 200 ng/mL VEGF165 (Genscript) and 2 μ M forskolin (Sigma-Aldrich)) starting on day 4. Transfection:Article Title: Characterization of an induced pluripotent stem cell line NCHi025-A from a 1-year-old female with pulmonary stenosis harboring a heterozygous HAND2 variant. Article Snippet: HAND2 is a transcription factor that plays a vital role in the development of the heart, limbs, and pharyngeal arch.. Functional defects in HAND2 have been shown to cause congenital malformations in the extremities of the body and the heart.. NCHi025-A iPSC line was generated from a 1-year-old female with pulmonary stenosis and harbors a novel de novo heterozygous variant of uncertain significance within HAND2 (NM_021973.3; c.247delG; p.Val83CysfsTer16). Article Title: No relationship between non-IgE-mediated mechanisms (complement activation or direct activation of mast cells and basophils) during diclofenac etalhyaluronate (SI-613/ONO-5704)-induced anaphylaxis Article Snippet: In brief, cells were cultured in StemPro-34 SFM medium supplemented with 2 mM Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation Article Snippet: The organoids were subsequently cultured in StemPro-34 SFM medium (10639011, Thermo Fisher Scientific) supplemented with 2% Article Title: Characterization of an induced pluripotent stem cell line (NCHi024-A) from a male infant with bicuspid aortic valve. Article Snippet: PBMCs were isolated and cultured for one week in StemPro-34 SFM medium (Thermo Fisher Scientific) supplemented with 100 ng/mL SCF (PeproTech), 20 ng/mL IL-3 (PeproTech), 100 ng/mL Article Title: Restoring mitochondrial function promotes hematopoietic reconstitution from cord blood following cryopreservation-related functional decline Article Snippet: After 24 h, the H5100 medium was replaced 630 with 200 μl erythro-myeloid differentiation medium containing StemPro-34 SFM 631 medium (Thermo Fisher) supplemented with the following cytokines (all from 632 Peprotech): SCF (100 ng/ml), Flt3-L (20 ng/ml), TPO (100 ng/ml), IL-6 (50 ng/ml), IL-633 3 (10 ng/ml), IL-11 (50 ng/ml), GM-CSF (20 ng/ml), IL-2 (10 ng/ml), IL-7 (20 ng/ml), 634 and EPO (3 units/ml), as well as 1% Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation. Article Snippet: The organoids were subsequently cultured in StemPro-34 SFM medium (10639011, Thermo Fisher Scientific) supplemented with 2% Article Title: Restoring mitochondrial function promotes hematopoietic reconstitution from cord blood following cryopreservation-related functional decline Article Snippet: After 24 hours, the H5100 medium was replaced with 200 μL erythro-myeloid differentiation medium containing StemPro-34 SFM medium (Thermo Fisher Scientific) supplemented with the following cytokines (all from Peprotech): SCF (100 ng/mL); FLT-3L (20 ng/mL); TPO (100 ng/mL); IL-6 (50 ng/mL); IL-3 (10 ng/mL); IL-11 (50 ng/mL); GM-CSF (20 ng/mL); IL-2 (10 ng/mL); IL-7 (20 ng/mL); and EPO (3 units/mL), as well as 1% Article Title: Adenine base editing rescues pathogenic phenotypes in tissue engineered vascular model of Hutchinson-Gilford progeria syndrome Article Snippet: To differentiate iPSCs to viECs, the cells were cultured in viEC induction media (Stempro-34 SFM medium (Thermo Fisher) supplemented with 200 ng/mL VEGF165 (Genscript) and 2 μ M forskolin (Sigma-Aldrich)) starting on day 4. Recombinant:Article Title: Characterization of an induced pluripotent stem cell line NCHi025-A from a 1-year-old female with pulmonary stenosis harboring a heterozygous HAND2 variant. Article Snippet: HAND2 is a transcription factor that plays a vital role in the development of the heart, limbs, and pharyngeal arch.. Functional defects in HAND2 have been shown to cause congenital malformations in the extremities of the body and the heart.. NCHi025-A iPSC line was generated from a 1-year-old female with pulmonary stenosis and harbors a novel de novo heterozygous variant of uncertain significance within HAND2 (NM_021973.3; c.247delG; p.Val83CysfsTer16). Article Title: No relationship between non-IgE-mediated mechanisms (complement activation or direct activation of mast cells and basophils) during diclofenac etalhyaluronate (SI-613/ONO-5704)-induced anaphylaxis Article Snippet: In brief, cells were cultured in StemPro-34 SFM medium supplemented with 2 mM Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation Article Snippet: The organoids were subsequently cultured in StemPro-34 SFM medium (10639011, Thermo Fisher Scientific) supplemented with 2% Article Title: Characterization of an induced pluripotent stem cell line (NCHi024-A) from a male infant with bicuspid aortic valve. Article Snippet: PBMCs were isolated and cultured for one week in StemPro-34 SFM medium (Thermo Fisher Scientific) supplemented with 100 ng/mL SCF (PeproTech), 20 ng/mL IL-3 (PeproTech), 100 ng/mL Article Title: Restoring mitochondrial function promotes hematopoietic reconstitution from cord blood following cryopreservation-related functional decline Article Snippet: After 24 h, the H5100 medium was replaced 630 with 200 μl erythro-myeloid differentiation medium containing StemPro-34 SFM 631 medium (Thermo Fisher) supplemented with the following cytokines (all from 632 Peprotech): SCF (100 ng/ml), Flt3-L (20 ng/ml), TPO (100 ng/ml), IL-6 (50 ng/ml), IL-633 3 (10 ng/ml), IL-11 (50 ng/ml), GM-CSF (20 ng/ml), IL-2 (10 ng/ml), IL-7 (20 ng/ml), 634 and EPO (3 units/ml), as well as 1% Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation. Article Snippet: The organoids were subsequently cultured in StemPro-34 SFM medium (10639011, Thermo Fisher Scientific) supplemented with 2% Article Title: Restoring mitochondrial function promotes hematopoietic reconstitution from cord blood following cryopreservation-related functional decline Article Snippet: After 24 hours, the H5100 medium was replaced with 200 μL erythro-myeloid differentiation medium containing StemPro-34 SFM medium (Thermo Fisher Scientific) supplemented with the following cytokines (all from Peprotech): SCF (100 ng/mL); FLT-3L (20 ng/mL); TPO (100 ng/mL); IL-6 (50 ng/mL); IL-3 (10 ng/mL); IL-11 (50 ng/mL); GM-CSF (20 ng/mL); IL-2 (10 ng/mL); IL-7 (20 ng/mL); and EPO (3 units/mL), as well as 1% Article Title: Adenine base editing rescues pathogenic phenotypes in tissue engineered vascular model of Hutchinson-Gilford progeria syndrome Article Snippet: To differentiate iPSCs to viECs, the cells were cultured in viEC induction media (Stempro-34 SFM medium (Thermo Fisher) supplemented with 200 ng/mL VEGF165 (Genscript) and 2 μ M forskolin (Sigma-Aldrich)) starting on day 4. Knock-Out:Article Title: Characterization of an induced pluripotent stem cell line NCHi025-A from a 1-year-old female with pulmonary stenosis harboring a heterozygous HAND2 variant. Article Snippet: HAND2 is a transcription factor that plays a vital role in the development of the heart, limbs, and pharyngeal arch.. Functional defects in HAND2 have been shown to cause congenital malformations in the extremities of the body and the heart.. NCHi025-A iPSC line was generated from a 1-year-old female with pulmonary stenosis and harbors a novel de novo heterozygous variant of uncertain significance within HAND2 (NM_021973.3; c.247delG; p.Val83CysfsTer16). Article Title: No relationship between non-IgE-mediated mechanisms (complement activation or direct activation of mast cells and basophils) during diclofenac etalhyaluronate (SI-613/ONO-5704)-induced anaphylaxis Article Snippet: In brief, cells were cultured in StemPro-34 SFM medium supplemented with 2 mM Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation Article Snippet: The organoids were subsequently cultured in StemPro-34 SFM medium (10639011, Thermo Fisher Scientific) supplemented with 2% Article Title: Characterization of an induced pluripotent stem cell line (NCHi024-A) from a male infant with bicuspid aortic valve. Article Snippet: PBMCs were isolated and cultured for one week in StemPro-34 SFM medium (Thermo Fisher Scientific) supplemented with 100 ng/mL SCF (PeproTech), 20 ng/mL IL-3 (PeproTech), 100 ng/mL Article Title: Restoring mitochondrial function promotes hematopoietic reconstitution from cord blood following cryopreservation-related functional decline Article Snippet: After 24 h, the H5100 medium was replaced 630 with 200 μl erythro-myeloid differentiation medium containing StemPro-34 SFM 631 medium (Thermo Fisher) supplemented with the following cytokines (all from 632 Peprotech): SCF (100 ng/ml), Flt3-L (20 ng/ml), TPO (100 ng/ml), IL-6 (50 ng/ml), IL-633 3 (10 ng/ml), IL-11 (50 ng/ml), GM-CSF (20 ng/ml), IL-2 (10 ng/ml), IL-7 (20 ng/ml), 634 and EPO (3 units/ml), as well as 1% Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation. Article Snippet: The organoids were subsequently cultured in StemPro-34 SFM medium (10639011, Thermo Fisher Scientific) supplemented with 2% Article Title: Restoring mitochondrial function promotes hematopoietic reconstitution from cord blood following cryopreservation-related functional decline Article Snippet: After 24 hours, the H5100 medium was replaced with 200 μL erythro-myeloid differentiation medium containing StemPro-34 SFM medium (Thermo Fisher Scientific) supplemented with the following cytokines (all from Peprotech): SCF (100 ng/mL); FLT-3L (20 ng/mL); TPO (100 ng/mL); IL-6 (50 ng/mL); IL-3 (10 ng/mL); IL-11 (50 ng/mL); GM-CSF (20 ng/mL); IL-2 (10 ng/mL); IL-7 (20 ng/mL); and EPO (3 units/mL), as well as 1% Article Title: Adenine base editing rescues pathogenic phenotypes in tissue engineered vascular model of Hutchinson-Gilford progeria syndrome Article Snippet: To differentiate iPSCs to viECs, the cells were cultured in viEC induction media (Stempro-34 SFM medium (Thermo Fisher) supplemented with 200 ng/mL VEGF165 (Genscript) and 2 μ M forskolin (Sigma-Aldrich)) starting on day 4. Derivative Assay:Article Title: Characterization of an induced pluripotent stem cell line NCHi025-A from a 1-year-old female with pulmonary stenosis harboring a heterozygous HAND2 variant. Article Snippet: HAND2 is a transcription factor that plays a vital role in the development of the heart, limbs, and pharyngeal arch.. Functional defects in HAND2 have been shown to cause congenital malformations in the extremities of the body and the heart.. NCHi025-A iPSC line was generated from a 1-year-old female with pulmonary stenosis and harbors a novel de novo heterozygous variant of uncertain significance within HAND2 (NM_021973.3; c.247delG; p.Val83CysfsTer16). Article Title: No relationship between non-IgE-mediated mechanisms (complement activation or direct activation of mast cells and basophils) during diclofenac etalhyaluronate (SI-613/ONO-5704)-induced anaphylaxis Article Snippet: In brief, cells were cultured in StemPro-34 SFM medium supplemented with 2 mM Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation Article Snippet: The organoids were subsequently cultured in StemPro-34 SFM medium (10639011, Thermo Fisher Scientific) supplemented with 2% Article Title: Characterization of an induced pluripotent stem cell line (NCHi024-A) from a male infant with bicuspid aortic valve. Article Snippet: PBMCs were isolated and cultured for one week in StemPro-34 SFM medium (Thermo Fisher Scientific) supplemented with 100 ng/mL SCF (PeproTech), 20 ng/mL IL-3 (PeproTech), 100 ng/mL Article Title: Restoring mitochondrial function promotes hematopoietic reconstitution from cord blood following cryopreservation-related functional decline Article Snippet: After 24 h, the H5100 medium was replaced 630 with 200 μl erythro-myeloid differentiation medium containing StemPro-34 SFM 631 medium (Thermo Fisher) supplemented with the following cytokines (all from 632 Peprotech): SCF (100 ng/ml), Flt3-L (20 ng/ml), TPO (100 ng/ml), IL-6 (50 ng/ml), IL-633 3 (10 ng/ml), IL-11 (50 ng/ml), GM-CSF (20 ng/ml), IL-2 (10 ng/ml), IL-7 (20 ng/ml), 634 and EPO (3 units/ml), as well as 1% Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation. Article Snippet: The organoids were subsequently cultured in StemPro-34 SFM medium (10639011, Thermo Fisher Scientific) supplemented with 2% Article Title: Restoring mitochondrial function promotes hematopoietic reconstitution from cord blood following cryopreservation-related functional decline Article Snippet: After 24 hours, the H5100 medium was replaced with 200 μL erythro-myeloid differentiation medium containing StemPro-34 SFM medium (Thermo Fisher Scientific) supplemented with the following cytokines (all from Peprotech): SCF (100 ng/mL); FLT-3L (20 ng/mL); TPO (100 ng/mL); IL-6 (50 ng/mL); IL-3 (10 ng/mL); IL-11 (50 ng/mL); GM-CSF (20 ng/mL); IL-2 (10 ng/mL); IL-7 (20 ng/mL); and EPO (3 units/mL), as well as 1% Article Title: Adenine base editing rescues pathogenic phenotypes in tissue engineered vascular model of Hutchinson-Gilford progeria syndrome Article Snippet: To differentiate iPSCs to viECs, the cells were cultured in viEC induction media (Stempro-34 SFM medium (Thermo Fisher) supplemented with 200 ng/mL VEGF165 (Genscript) and 2 μ M forskolin (Sigma-Aldrich)) starting on day 4. Mutagenesis:Article Title: Characterization of an induced pluripotent stem cell line NCHi025-A from a 1-year-old female with pulmonary stenosis harboring a heterozygous HAND2 variant. Article Snippet: HAND2 is a transcription factor that plays a vital role in the development of the heart, limbs, and pharyngeal arch.. Functional defects in HAND2 have been shown to cause congenital malformations in the extremities of the body and the heart.. NCHi025-A iPSC line was generated from a 1-year-old female with pulmonary stenosis and harbors a novel de novo heterozygous variant of uncertain significance within HAND2 (NM_021973.3; c.247delG; p.Val83CysfsTer16). Article Title: No relationship between non-IgE-mediated mechanisms (complement activation or direct activation of mast cells and basophils) during diclofenac etalhyaluronate (SI-613/ONO-5704)-induced anaphylaxis Article Snippet: In brief, cells were cultured in StemPro-34 SFM medium supplemented with 2 mM Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation Article Snippet: The organoids were subsequently cultured in StemPro-34 SFM medium (10639011, Thermo Fisher Scientific) supplemented with 2% Article Title: Characterization of an induced pluripotent stem cell line (NCHi024-A) from a male infant with bicuspid aortic valve. Article Snippet: PBMCs were isolated and cultured for one week in StemPro-34 SFM medium (Thermo Fisher Scientific) supplemented with 100 ng/mL SCF (PeproTech), 20 ng/mL IL-3 (PeproTech), 100 ng/mL Article Title: Restoring mitochondrial function promotes hematopoietic reconstitution from cord blood following cryopreservation-related functional decline Article Snippet: After 24 h, the H5100 medium was replaced 630 with 200 μl erythro-myeloid differentiation medium containing StemPro-34 SFM 631 medium (Thermo Fisher) supplemented with the following cytokines (all from 632 Peprotech): SCF (100 ng/ml), Flt3-L (20 ng/ml), TPO (100 ng/ml), IL-6 (50 ng/ml), IL-633 3 (10 ng/ml), IL-11 (50 ng/ml), GM-CSF (20 ng/ml), IL-2 (10 ng/ml), IL-7 (20 ng/ml), 634 and EPO (3 units/ml), as well as 1% Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation. Article Snippet: The organoids were subsequently cultured in StemPro-34 SFM medium (10639011, Thermo Fisher Scientific) supplemented with 2% Article Title: Restoring mitochondrial function promotes hematopoietic reconstitution from cord blood following cryopreservation-related functional decline Article Snippet: After 24 hours, the H5100 medium was replaced with 200 μL erythro-myeloid differentiation medium containing StemPro-34 SFM medium (Thermo Fisher Scientific) supplemented with the following cytokines (all from Peprotech): SCF (100 ng/mL); FLT-3L (20 ng/mL); TPO (100 ng/mL); IL-6 (50 ng/mL); IL-3 (10 ng/mL); IL-11 (50 ng/mL); GM-CSF (20 ng/mL); IL-2 (10 ng/mL); IL-7 (20 ng/mL); and EPO (3 units/mL), as well as 1% Article Title: Adenine base editing rescues pathogenic phenotypes in tissue engineered vascular model of Hutchinson-Gilford progeria syndrome Article Snippet: To differentiate iPSCs to viECs, the cells were cultured in viEC induction media (Stempro-34 SFM medium (Thermo Fisher) supplemented with 200 ng/mL VEGF165 (Genscript) and 2 μ M forskolin (Sigma-Aldrich)) starting on day 4. |